2s. For attenuation las in the try operon) to work in eakaryotes: A. RNA process

Question

2s. For attenuation las in the try operon) to work in eakaryotes: A. RNA processing would need to be in the cytoplasm B. would need to be c RNA processing need to be done by mere bacterial RNA pols (pol 1 and pol D. there would need to be room in the nucleus for protein folding. E translation would need to start in the nudeus. 30 The transcription factors Tupl-Migl control GALA activity byi A binding to silenar sites to up regulate tra by deacylating local histones which up regulate local genes B. binding to enhancer sites to regulate transcription by acylating local histones which down regulate local genes C binding to silencer sites to regulate transcription by acylating local histones which down regulate local genes. D binding to enhancer sites to up regulate transcription by deacylating local histones which up regulate local genes. E. binding to silencer sites to regulate transcripcion by deacylating local histones which down regulate local genes.

Explanation / Answer

Question 29. As you know that the attenuation mechanism as it works in the case of trp operon is dependent on two factors: 1) the secondary hairpin structure formation in the recently formed nascent RNA and 2) on the ribosome binding to this nascent RNA.

Both factors need to work simultaneously to perform this attenuation. Therefore, it is clear that for this attenuation to work, the transcription and translation need to occur simultaneously. Now we know that in Eukaryotes transcription occurs in nucleus first and then RNA moves out of the nucleus and the translation occurs in the cytosol.

Now let us evaluate the options

A) RNA processing anyways occurs in nucleus in eukaryotes and this can not make the co-occurrence of transcription and translation. So Wrong

B) Being Eukaryotic genes to be polycistronic does not matter to the co-occurrence of transcription and translation. So Wrong

C) RNA processing has no role to play in co-occurrence of transcription and translation.  So Wrong

D) Space in nucleus is no way related to co-occurrence of transcription and translation.  So Wrong

E) Yes, if the translation can start in nucleus which usually occurs in the cytosol in eukaryotes, this could lead to the co-occurrence of transcription and translation. So Correct

Question 30. The Tup1-Mig1 regulate the gene expression of the GAL4 by working in a joint venture. The Mig1 protein has the DNA binding site using which this can bind to the silencer DNA sequence present upstream of the GAL4 gene. After binding, this recruits the Tup1 repressor complex which contains multiple histone deacetylases as well. Now these histone deacetylases deacetylate the histones which lead to the compaction of the nucleosome structures leading to overall gene silencing. Now let us evaluate the options:

A) Binding to silencer sequence can not lead to upregulation. So Wrong

B) Acylation of histones leads to up-regulation not down-regulation. So Wrong

C) Again, acylation of histones leads to up-regulation not down-regulation. So Wrong

D) Deacylation can not upregulate the gene expression. So Wrong.

E) Correct, because bind to silencer lead to transcription regulation and the following deacylation will lead to the down-regulation of the gene expression. So Correct

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