knockout mice (Figure 3G-N) of the myelin sneau animals. Discuss the compared to
ID: 223184 • Letter: K
Question
knockout mice (Figure 3G-N) of the myelin sneau animals. Discuss the compared to the wild-type structure results presented in the aforementioned panels. (4 points) 7) Autophagy is the process by which a cell degrades unnecessary or dysfunctional materials. This mechanism is characterized by a conversion of LC3-l protein to Lc3-ll protein. Based on this information, do the results presented in Figure 30-P make sense? Explain your answer. (3 points) 8) The authors concluded that cisd2 is localized to the outer mitochondrial membrane. Explain the experimental evidence in Figure 4 that supports their assertions. In your response, explain which portion of the Cisd2 protein is necessary for proper localization to the mitochondria. (5 points) 9) Why did the investigators apply glutamate-malate and ADP to mitochondria isolated from the Cisd2 knockout mice? They noticed an increase in oxygen content and a decrease in oxygen consumption within these deficient mice compared to wild-type animals after the aforementioned additions. What do these results mean in the context of aerobic respiration in the mice lacking Cisd2? (4 points) 10) In Figure 5, the researchers performed glucose (Figure 5C-D) and insulin tolerance (Figure E) tests to determine if the Cisd2 knockout mice were diabetic. Discuss the results presented in the three panels and comment a to whether these mice are considered to be diabetic. (6 points)Explanation / Answer
During autophagy, the conversion of LC3 I into LC3 II takes place. So, there should be increase in LC3 II protein when detected in western blotting and other tests. In O diagram we see the LC3 II protein is more detected than LC3 I.
And in the P graph, the ratio LC3 II/LC3 I is showing an increase between a period of 12 wk to 24 wk. It means the amount of LC3 II protein has increased. It is due to mechanism of autophagy.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.