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(a 1 x 106 (a) 1 x 10 high contact x 105 1 x 105 ow contact 1 x 104 1 x 103 1 x

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Question

(a 1 x 106 (a) 1 x 10 high contact x 105 1 x 105 ow contact 1 x 104 1 x 103 1 x 10 x 102 experimental treatment (b) 1x10 encounter test 1 x 10 (b) 1x10 1 x 106 1 x 10 1x105 1 x 104 1 x 104 1 x 103 1 x 10 10 15 20 30 1 x 102 time (min) Figure 5. (a) Box plot representation of the variation of bio- 1 x 10 luminescence intensity recorded for 0 or 1 snail H. brasiliana 10 30 (H.b.), combined or separated from the amphipod time (min) C. uncinata (C.u.). Brackets indicate when separation with divider was present, while treatments under the same line Figure 4. (a Box plot representation of the variation ofbio- luminescence intensity recorded for high- and low-contact represent s paired conditions. (b) Bioluminescence organisms and controls. (b) Bioluminescence production production profile of H. brasiliana when exposed to the amphi- profile of H. brasiliana when exposed to one high-contact pod C. uncinata initially separated (dark grey line) or initially polychaete lack line) and two low-contact conspecifics combined (black line) using a plastic divider. The light grey line represents background control of the instrument. grey line; see electronic supplementary material, figure S3 for expanded flash characteristics). RLU, relative light units characterize the functional aspects of bioluminescence in H brasiliana. In situ, planaxid snails often cluster together in high In comparison, the well-studied terrestrial biolumines- numbers in moist crevices or under rocks during low cent pulmonate snail Dyakia striata (Gray, 1834) emits light from a glandular organ located on the head, which tide, giving rise to their common name of "clusterwinks Although speculative, it is reasonable to assume that a s expressed as extended flashes/glows up to 6 s ong large group of snails that flash when threatened would (541. Light production in D. striata occurs mainly when further deter predation, thus enhancing the "flash bulb the foot is extended outside the shell and in response to effect that might temporarily blind nocturnal, visual pre- ght stimulation, while being inhibited by mechanical dators (9,50]. It is also possible that the light production stimulation [55,56]. The function of light emission in D. striata remains uncertain a communication role has acts as an aposematic signal [51-53], but this is less prob- able considering the gregarious and cryptic behaviour of been proposed [57], but is complicated, and hypotheses H. brasiliana. Further experiments are needed to fully regarding defence and links to sexual maturity have

Explanation / Answer

Figure 4:

Figure 5:

Therefore, we can conclude that mechanical disturbance caused by high contact organisms triggered enhanced bioluminescence in the snail.