Surface Marker Immonostaining My answers are incorrect. please help! QUESTION 1

Question

Surface Marker Immonostaining
My answers are incorrect. please help! QUESTION 1 Why are cells kept at 4 degrees centigrade during antibody staining? To minimize surface marker endocytosis to minimize non-specific interactions of the antibody a and b none of the above QUESTION 2 What fluorochrome was the antibody used to stain the surface marker conjugated to? O Phycoerythrin (red) O FITC (green APC none of the above QUESTION 3 What surface marker was targeted during the antibody staining lab? CD4 CD8 CD14 On none of the above

Explanation / Answer

ques1 - d) none of the above

cells are kept at 4 degree centrigade to maintain vailbility of cells and avod receptor internalization

ques2 - d) none of the above

the flourochrome used to stain the antibody is the tandem dyes

ques3 - a) CD4

This is used as marker

ques4 - a) to remove the excess protein or antibodues that interfere with the process

ques5 - true

Yes, it is easier to resuspend pellet when sitting in small volume of liquid.

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