How did Alfred Hershey and Martha Chase contribute to our understanding of DNA?

Question

How did Alfred Hershey and Martha Chase contribute to our understanding of DNA? a. Used X-ray diffraction to examine the structure of DNA b. Determined that DNA contains four different nitrogenous bases c. Found that "the transforming principle" is destroyed by enzymes that hydrolyze DNA d. Found that the phosphorus-containing components are the genetic material of phages e. Discovered "the transforming principle" that could genetically alter bacteria With respect to their 3' and 5' ends, the two polynucleotide chains of a double-stranded DNA molecule are a. antiparallel. b. isotopes. c. methylated. d. complementary. e. nitrogenous. If the sequence of one strand of DNA is 5'-GCTAGCGTCG-3', what is the sequence of the complementary strand a. 3'-GCTAGCGTCG-5' b. 5'-GCTGCGATCG-3' c. 3'-CGATCGCAGC-5' d. 5'-CGATCGCAGC-3' e. 5'-CGAUCGCAGC-3' You are studying a small eukaryotic gene of about 2000 bp in length. Estimate how many copies of histone H4 y along this region of the chromosome. a. 10 b. 20 c. 40 d. 80 e. 100 Where would you expect to find the variant histone CenH3? a. telomere b. euchromatin c centromere d. mitochondria e. chloroplast

Explanation / Answer

(10)

Remember, A pairs with T and G pairs with C. Go through the original 5 to 3 sequence pairing each A with T and each C with G. Keep in mind that the complementary strand will read from left to right in the 3 to 5 direction. Therefore, the complementary strand starts with 3’ and ends with 5’.

Original strand : 5 GCTAGCGTCG 3

Complementary strand: 3 CGATCGCAGC 5

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