8. In E. coli, Hfr strains occur when the F plasmid integrates into the circular

Question

8. In E. coli, Hfr strains occur when the F plasmid integrates into the circular chromosome. Remember that the F plasmid can integrate into the chromosome in various locations. During subsequent conjugation events, not only will F plasmid DNA be transferred to a recipient cell, but chromosomal DNA can also be transferred. In one experiment, four E. coli Hfr strains donate the following markers, shown in the order donated:

Strain 1: M Z X W C

Strain 2: L A N C W

Strain 3: A L B R U

Strain 4: Z M U R B

All these Hfr strains are derived from the same F+ strain. Draw the chromosome of the original F+ E. coli strain.

Explanation / Answer

Overlapping method used to identify this one. Here F plasmid is circular:

M. Z. X. W. C

W. C. N. A. L

A. L. B. R. U

So the chromosome sequence is: M. Z. X. W. C. N. A. L. B. R. U

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