You are given two samples of DNA, one from Clostridium perfringens and the other

Question

You are given two samples of DNA, one from Clostridium perfringens and the other from Mycobacterium tuberculosis. Unfortunately, the labels on the tubes of DNA were accidently removed, and you do not know which DNA sample belongs to which organism. As you were doing some work with Escherichia coli in the lab, you also have some of its DNA available as well. The % (G+C) value for each of the organisms is known (C. perfringens, 27%; M. tuberculosis, 67%; E. coli, 50%). Based upon this information, how could you quickly determine the source of the DNA for your two samples?

Explanation / Answer

out of the three organisms the one with the highest G+C content in their genome will have the highest melting temprature and the lowest G+C content will have the lowest melting temperature. this is because of the three hydrogen bonds present between G and C.

so we can do the melting temperature experiment to find out the different melting temperature of the organisms.

according to their GC content M.tuberculosis will have the highest melting temperature and C.pefringes will have the lowest melting temperature.

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