Gene targeting can be used to study the function of a gene. Which of the followi
ID: 226945 • Letter: G
Question
Gene targeting can be used to study the function of a gene. Which of the following statements describing gene targeting are true? SELECT ALL POSSIBLE ANSWERS
I. The use of gene targeting is limited to model organisms, such as rats, where gene targeting methods are well established.
II. Gene targeting can be used to introduce a point mutation into an existing gene to observe what happens when the gene is modified.
III. Gene targeting to delete a gene can be performed on an adult organism so that the before and after effects can be observed.
IV. Gene targeting can be used to turn off a gene to observe what happens when the gene is not expressed.
Explanation / Answer
Answer
Gene trageting is a genetic technique which uses homologous recombination to change an endogenous gene. This method can be used to delet or add a gene, remove exons, and introduce point mutations. Gene targeting requires construction of a specific vector for ech gene of interest. However, it can be used for any gene, regardless of transcriptional activity or gene size.
The following statements are True regarding Gene Targeting:
1. The use of gene targeting is limited to model, organisms, such as rats, where gene targeting methods are well established. Model organisms are non-human species that are used in the laboratory to help understand the biological processes. These organisms are easy to maintain and breed in the laboratory settings and has particluar experimental advantages.
2. Gene targeting can be used to introduce a point mutation into an existing gene to observe what happens when the gene is modified. Through gene targeting researches can introduce point mutations into an existing gene and can examine the modifications in the gene after adding a point mutation.
4. Gene targeting can be used to turn off a gene to observe what happens when the gene is not expressed. Gene targetimng allows the researchers to delete a gene, remove exons in the model organisms. Gene targeting is widely used to study human genetic diseases by removing (knocking out)/turning off or adding (knocking in), specific mutations or genes of intrest to a variety of models and then study the gene functioning.
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