Genetics Question. You are studying a protein that contains the amino acid selen

Question

Genetics Question.

You are studying a protein that contains the amino acid selenocysteine. To understand the protein better, you place the gene for this protein into a plasmid, so that you can control the expression of the gene in cells. When you look at the protein expressed from the plasmid, however, you are surprised to find that the protein encoded from the plasmid is shorter than the protein encoded from the genome. Why? You included the SECIS, and therefore the UGA functioned as a stop codon rather than a codon for selenocystelne. You likely placed the Incorrect gene Into the plasmid. Selenocystelne can only be included in proteins encoded by the genome, therefore the UGA functioned as a stop codon, rather than a codon for selenocystelne. You forgot to Include the SECIS. and therefore UGA functioned as a stop codon rather than a codon for selenocystelne.

Explanation / Answer

Q: you are studying a protein that contains the amino acid selenocysteine. To understand the protein better, you place the gene for this protein into a plasmid, so that you can control the expression of the gene in cells. When you look at the protein expressed from the plasmid, however, you are surprised to find that the protein encoded from the plasmid is shorter than the protein encoded from the genome. Why?

Answer: You forgot to include the SECIS, and therefore UGA functioned as a stop codon rather then a codon for selenocysteine.

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