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Recall the steps whereby recombinant DNA technology was used to make insulin. Th

ID: 227152 • Letter: R

Question

Recall the steps whereby recombinant DNA technology was used to make insulin. The word 'recombinant' refers to the formation of A. fragments of human DNA. B. hybrid plasmids. C. fragments of bacterial RNA. D. restriction enzymes. Before 1940, why did so many researchers think the genetic material was protein? A, chromosomes contain both DNA and protein. B. proteins are made from 20 possible amino acids, so should be able to carry more information than DNA, which is made from only 4 bases. C. Chromosomes were thought to contain model molecules for each of the body's proteins. D. All of the above. Which discovery about the structure of DNA was made AFTER all the others? A. DNA, not protein, is the genetic material B. Specific base pains face inward B. Chargaff's rule. C. DNA has a helical structure. What did Rosalind Franklin use to measure the size and angles of DNA's helix? A. Xray diffraction photos. B. Radioactive labels in DNA's backbone. C. 3-D models that looked like "tinker toys". D. Chargaff's ruler. If you cross a homozygous dominant with a homozygous recessive, what percentage of the offspring will be heterozygous A 25% B. 50% C. 75% D. 100% The allele for freckles (F) is dominant to the allele for no freckles (f). A man with freckles marries a woman who also has freckles, but their first born child has no freckles. What are the chances their next child will have freckles? A. 100% B. 75% C. 50% D. 25% Mendel's Law of Segregation make sense in light of what we now know about the movements of chromosomes during A. mitosis B. meiosis C. transcription D. translation In human blood types, the allele for Rh+ is dominant. For which one of these phenotypes is it impossible to have the baby born "blue" with Rh disease? [No Punnet square needed!] A. The mother is Rh positive, B. The father is Rh positive. C. The baby is Rh positive. D. The mother is Rh negative. Which of these diseases is caused by non- disjunction of chromosomes during meiosis? A. Huntington's disease. B. Trisomy 21 C. Cystic fibrosis. D. Red-green colorblindness. In humans, colorblindness is sex-linked and recessive. Consider a woman with normal vision whose father was colorblind. What are the chances that this woman is a carrier for the colorblindness? No Punnett square needed!] A. 0% B. 25% C. 50% D. 100%

Explanation / Answer

21. Hybrid plasmids- human insulin gene inserted into a bacterial plasmid and this plasmid returned to the bacterium and underwent fermentation process to produce insulin.

22. B. protein made from 20 different aminoacids, so should be able t carry more information than DNA which is made from only 4 bases. Also proteins were macromolecules with great heterogenicity.

23. DNA has a helical structure- Watson and Crick- 1953