Problem 21.13 Consider the A bacteriophage DNA molecule as shown in the figure b

Question

Problem 21.13 Consider the A bacteriophage DNA molecule as shown in the figure below. Total digestion with the two restriction endonucleases used does not allow unambiguous placement of restriction sites, as shown in the map. BamHI sites A DNA BamHI segments A EcoRI sites A DNA EcoRI segments o 30 5 DNA length, kb Restriction maps of cleavage sites for each enzyme on the DNA molecule. By convention, the fragments are assigned letters as shown. In this experiment the 12.1-kb fragment in the BamHI digest resulted from linking the A and F (terminal fragments by base pairing at their cohesive ends.

Explanation / Answer

21.13 Part A -

its answer will be option C as in that case only we will be able to get all the fragment of DNA upon digestion with BamHI and EcoRI

21.11 Part A.

option D is correct.

DNA present in the histone is also supercoiled. So when topoisomerase treatment is given and then histone are removed we will still have supercoiled DNA

Sorry for my mistake its answer will option A

Related Questions

Navigate

• Browse (All)
• Browse P
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.