Why is Tm related to base composition? (Hint: Tm refers to temperature of meltin

Question

Why is Tm related to base composition? (Hint: Tm refers to temperature of melting
A. The fewer hydrogen bonds between bases, The higher the temperature needed to separate the pair.
B. The more hydrogen bonds between bases, The higher the temperature needed to separate the pair
C. Neither, Tm is related to UV light absorption
D. Neither, TM is related to the number of phosphate groups presented in the double-stranded DNA molecule

Why is Tm related to base composition? (Hint: Tm refers to temperature of melting
A. The fewer hydrogen bonds between bases, The higher the temperature needed to separate the pair.
B. The more hydrogen bonds between bases, The higher the temperature needed to separate the pair
C. Neither, Tm is related to UV light absorption
D. Neither, TM is related to the number of phosphate groups presented in the double-stranded DNA molecule


A. The fewer hydrogen bonds between bases, The higher the temperature needed to separate the pair.
B. The more hydrogen bonds between bases, The higher the temperature needed to separate the pair
C. Neither, Tm is related to UV light absorption
D. Neither, TM is related to the number of phosphate groups presented in the double-stranded DNA molecule

Explanation / Answer

Ans....(B)....The more hydrogen bonds between bases,The higher the temperature needed to separate the pair.

Melt temperature varies with base composition for instance a greater GC content typically increases Tm because more hydrogen bonds need disrupting in GC base-pairs.

Tm of the hybrid is an important factor for consideration,the Tm of a hybrid depends on its composition,DNA-DNA hybrids have the lowest Tm and RNA-RNA hybrids have the highest.

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