Using the following hypothesis from the Enzyme experiment: The rate of an enzyma
ID: 228607 • Letter: U
Question
Using the following hypothesis from the Enzyme experiment: The rate of an enzymatic reaction maybe increased by the enzyme concentration. What information would a reader need to know about enzymes to understand the independent variable and how it is related to the hypothesis? List 3 facts that would be required to understand why the independent variable for the experiment is enzyme concentration. independent variable pH level, dependent variable is the height of bubble reaction after hydrogen peroxide is added.
Explanation / Answer
#Let us first know what is independent and independent variables;
Independent variable-one that we can manipulate in a reaction(one that you can control)
Dependent variable-one that we want to measure(it is the result,not is in our control)
Illustration 1-suppose we want to know the sale of clothes by changing the prices in a shop.
changing price was independent variable (we can manipulate,and is in our control),but the sale of clothes is what we want to know/measure,it is not in our control);
illustration 2-we want to know oxygen consumption of particular enzyme at variable temperatures
here temperatures are independent variable and oxygen levels are dependent variables.
*Enzymes have a particular site called as active site ,for to reaction to get catalysed - the substrate should bind at enzyme active site.That means reaction process accelerates after substrate binded to active site of the enzyme.
in our hypothesis(above question) enzyme concentration is independent variable and rate of reaction is dependent variable.
-increaing enzyme concentration results in more enzyme molecules colliding with the substrate and more active sites binding takes place which leads to increase in enzymatic reaction initially.
although enzyme concentration is more ,but due to limited substrate(CONSTANT) no more substrate-enzyme interaction takes place at this point it acts as a limiting factor.
INCREASING ENZYME CONCENTRATION WILL INCREASE RATE OF REACTION UP TO CERTAIN CONCENTRATION,WHERE THE ENZYME CONCENTRATION IS NO LONGER THE LIMITING FACTOR
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