2. You\'ve isolated a novel enzyme, Sadase, that cleaves the substrate Sad into

Question

2. You've isolated a novel enzyme, Sadase, that cleaves the substrate Sad into the product Happy. During your study, you isolate two ligands, Schule and Lehrer. Happy produces a the following data looking at the change in absorbance in 2i s strong signalat 531 nm and you isolate th increasing concentrations of Sad Sad] VI1l (Sadase) V(2) (Sadase* V3) (Sadase Schule Lehrer 0.151 0.19 0.419 0.483 0.522 0.513 0.105 0.222 15 30 45 0.366 0.469 0.522 0.515 0.273 0.288 0.299 Determine if these ligands are positive or negative regulators. Additionally, determine the type of inhibition for any negative reguiators, Be sure to clearty show your analysis and explain your rationale, No credit Wll be given for answers without explanation MacBook Pro an F9 F1O F6 F7

Explanation / Answer

1.Ligands Schule and Lehrer are positive and negative regulators .

2.Binding of Schule ligand to sadase increases the attraction between sad to sadase . This can be seen from the observation .

3.As the concentration of Sadase + Schule increases , the Concentration of Sadase + sad also increases. Hence this is heterotropic positive allolestric regulator.

4.On the other hand , as the concentration of sadase + schule incraeses, the concentration of sadase + lehrer decreases.

5.Lehrer is negative regultor for sadase and it is competitive inhibitor for sad. As more number of active sites are occupied by Lehrer , sad could not bind to sadase.

6.Binding of Schule increaes affinity for sad in sadase and more number of active sites are occupied by sad producing happy.

7.The type of inhibition is competitive inhibition by negative regulator.

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