From the list, select the essential procedures to use in order to obtain washed

Question

From the list, select the essential procedures to use in order to obtain washed erythocytes. You won't necessarily employ all of the listed steps, and one or more instructions may be repeated.

A) obtain whole blood
B) dilute with isotonic buffer
C) dilute with hypotonic (dilute) buffer
D) resuspend pellet in dilute buffer
F) keep the pellet (store frozen)
H) centrifuge at high speed
I) resuspend pellet in isotonic buffer
L) centrifuge at low speed
S) discard the supernatant
W) wash (resuspend and recentrifuge) the pellet one or more times
X) discard the pellet

Place the methods in proper order by letter (please don't separate letters with commas or spaces From the list, select the essential procedures to use in order to obtain washed erythocytes. You won't necessarily employ all of the listed steps, and one or more instructions may be repeated.

A) obtain whole blood
B) dilute with isotonic buffer
C) dilute with hypotonic (dilute) buffer
D) resuspend pellet in dilute buffer
F) keep the pellet (store frozen)
H) centrifuge at high speed
I) resuspend pellet in isotonic buffer
L) centrifuge at low speed
S) discard the supernatant
W) wash (resuspend and recentrifuge) the pellet one or more times
X) discard the pellet

Place the methods in proper order by letter (please don't separate letters with commas or spaces From the list, select the essential procedures to use in order to obtain washed erythocytes. You won't necessarily employ all of the listed steps, and one or more instructions may be repeated.

A) obtain whole blood
B) dilute with isotonic buffer
C) dilute with hypotonic (dilute) buffer
D) resuspend pellet in dilute buffer
F) keep the pellet (store frozen)
H) centrifuge at high speed
I) resuspend pellet in isotonic buffer
L) centrifuge at low speed
S) discard the supernatant
W) wash (resuspend and recentrifuge) the pellet one or more times
X) discard the pellet

Place the methods in proper order by letter (please don't separate letters with commas or spaces

Explanation / Answer

C) dilute with hypotonic (dilute) buffer

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