Phenyl-Sepharose resin consiss of a phenyl group attached to a matrix of agarose

Question

Phenyl-Sepharose resin consiss of a phenyl group attached to a matrix of agarose. Suppose you need to use this chromatographic method to separate a mixture of polypeptides consisting of polyalanine, polyserine, polytryptophan and polylysine. Would you apply these polypeptides in a buffer containing high ionic strength or low ionic strength? What buffer would you choose (choose from one of "Good's buffers"(find a list online)? How would you elute the polypeptides, and what would be the order of elution?

Explanation / Answer

Phenyl Sepharose 6 Fast Flow (low sub) and. Phenyl ..... followed this up with new developments in agarose matrix design by introducing new .... ligands (alkyl or aryl groups) covalently attached to the gel matrix. ... S=Solute molecule ..... HIC Media Test Kit consists of five ready-to-use 1 ml plastic columns for screening

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