Using C1V1=C2V2, solve: 1) You have a stock solution containing 100mg/ml ampicil

Question

Using C1V1=C2V2, solve:

1) You have a stock solution containing 100mg/ml ampicillin. You need 500ml LB media containing ampicillin at 100ug/ml. How much ampicillin should you add?

2) You have a 50X solution of TAE buffer. You want 4L of a 1X solution of TAE buffer. How much TAE should you add? How much water?

3) You have a 1M stock solution of Tris-HCl. You need a 5ml of a 90mM Tris-HCl solution. How much stock should you add?

Here is what I got: 1) 0.5ml; 2) 0.08L; 3) 0.45mL
Are my answers correct? If not, can you please show work and explain how to set the problems up?

Explanation / Answer

Remember that a mg is 1000 ug. So you just dilute 1000-fold to get from 100 mg/ml to 100 ug/ml. I like to make stock solutions that are 1000X because there's basically no calculation involved when you use them. In this case, that woudl be 1 ml of your stock solution per liter. (And I always use 50 ug/ml - you can probably save on penicillin some if your'e just growing plasmids).

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