Which of the following is the best description of what might occur in a chain-te

Question

Which of the following is the best description of what might occur in a chain-termination sequencing reaction in which too little ddNTP is added?
Answer

A) When the concentration of ddNTP is too low, the frequency of incorporation of the ddNTP would also be low and therefore the shorter fragments of DNA would be overrepresented relative to the longer fragments.

B) It would not make any difference since the DNA polymerase does not differentiate between the ddNTP and dNTP nucleotides.

C) When the concentration of ddNTP is too low, the frequency of incorporation of the ddNTP would also be low and therefore the longer fragments of DNA would be overrepresented relative to the shorter fragments.

D) None of the fragments would be observable since the concentration of the ddNTP nucleotides is not correct.

Explanation / Answer

It would not make any difference since the DNA polymerase does not differentiate between the ddNTP and dNTP nucleotides.

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