whay is intact?????????? Vector unpacking is generally assumed to be neces- sary
ID: 252118 • Letter: W
Question
whay is intact??????????
Vector unpacking is generally assumed to be neces- sary for DNA release and gene expression, but the extent to which dissociation affects gene delivery is unclear For lipoplexes, it has been proposed that fusion of the cationic lipid with endosomal membrane lipids facili tates not only endosomal escape but also DNA release13. Polyplexes have been observed to localize intact to the nucleus, where they presumably undergo dissocia tion145. For certain polyplexes, mechanistic studies have reported slow vector unpacking as an explanation for decreased transfection efficiency16. Lipoplex-delivered plasmids are more efficiently expressed by nearly tenfold on the basis of protein expression per plasmid num ber in the nucleus - than polyplex-delivered plasmids, which might be a potential consequence of incomplete polyplex dissociation in the nucleus7.Explanation / Answer
Answer:- Some synthetic vectors are used for targeted delivery of anticancer agents intracellularly. These vectors are formed of a organized structure such as a micelle, liposome or a polymer. When this organized structure is attached with a DNA it is known as lipoplex or polyplex. If these polyplexes reach nucleus and integrate with it without unpacking its micelle like structure then it is known as the intact form. Here, the intact localization means the exact same thing. Intact localization to the target increases the efficiency of a polyplex.
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