264 Microinjection of DNA to Create Transgenic Mice This was a trial experiment

Question

264 Microinjection of DNA to Create Transgenic Mice This was a trial experiment of the technology involved and produced the world's fis fiest transgenic weghe 23 g 1. In the conbext of recombinant ONA technology. define the terms (b) Foreign DNA 2. Outline the basic principles involved in the production of a transgenic organism DNA an example of improvement in a commercial cröp brought about by the a of transgenvic techniques 4. Describe three human needs that have encouraged the development of transgenic techniques: S. Describe two advantages and one disadvantage of using viruses as vectors for gene delivery (a) Advantages (b) Disadvantage: 6. Explain the purpose behind the transgenic mice experiment (above): Photeeopying Prohibited

Explanation / Answer

1. certain Insect-resistant crops include genes from the soil bacterium Bacillus thuringiensis (Bt). The protein that is produced in the plant by the Bt gene is toxic to a group of targeted insects.

for example, European corn rootworm—but not to mammals.

So the use of these crops has increased the yield of crops.

2. a. transgenic techniques enable the insertion of foreign genes into embryos which in turn has contributed new tools for the production of new strains of animals that bear unique, beneficial genetic data.

b. transgenic techniques help in developing Genes to increase the content of amino acids in the seed which further increases the nutritional value, thereby decreasing the necessity for incorporating costly feed supplements.

c. transgenic techniques help to Achieve the least necessary growth in the total generation of global staple crops without additional increase in land under cultivation.

3. different viral vectors have different advantages and disadvantages for example:

retroviruses

Advantages:

Disadvantages

Herpesvirus:

Advantages

Disadvantages

4

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