Use the picture or scan of your SDS-PAGE separation of fish muscle proteins and

Question

Use the picture or scan of your SDS-PAGE separation of fish muscle proteins and measure the distance from the application well to each one of the molecular weight markers (KS) bands. The small proteins will move further down the gel. Use this table to identify the proteins and write down their migration distance:

the picture ^^

2. Comparison of protein composition. Use the picture or scan of your SDS-PAGE separation of fish muscle proteins and measure the distance from the application well to each one of the molecular weight markers (KS) bands. The small proteins will move further down the gel Use this table to identify the proteins and write down their migration distance: Molecular weight Distance migrated from well (mm) Protein (kDa) 1. Myosin-heavy chain 2. B-Galactosidase 3. Bovine serum albumin 4. Carbonic a 5. Soybean trypsin inhibitor 6. Lvsozyme 7. Aprotinin 203.0 135.0 86.0 41.5 33.4 19.5 8.0 drase

Explanation / Answer

distance migrated from well needs to be calculated using scale directly on the gel. if we measure the distance using gel image, it gives incorrect values. the approximate size of the image is 5 cm, which is not correct in real.

band for myosin heavy chain may be present in Hake lane

band for beta galactosidase may also be present in Hake lane

the band for bovine serum albumin may be present in catfish lane. however, the above three bands are not clear. they might be absent even

the bands for carbonic anhydrase is present in Trout, Bass and Swordfish lanes

soybean trypsin inhibitor may be present in swordfish lane

lysozyme is present in Hake lane

aprotinin is not clear. it may be present in flounder lane

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