What is hj? #3: Cell Yield Values under Variety of Growth Conditions Consider a

Question

What is hj?

#3: Cell Yield Values under Variety of Growth Conditions Consider a series of batch culture experiments performed using the bacterium Escherichia coli with glucose as the limiting substrate, and iron provided at different levels ranging from iron sufficient to iron deficient conditions. The data in the following table present the initial glucose level in the growth medium, the initial and final iron levels in the growth medium, and the initial and final cell concentrations for seven different batch culture experiments. Using this data, calculate the apparent yield of cell mass on glucose substrate for each batch, and then plot these values (dependent parameter) as a function of the initial level of iron provided (independent parameter, suggested plot with a logarithmic axis). Comment on the trend for eld on glucose as a function of the initial level of iron provided in the batch. Iron is an important procaryotes and eucaryotes. Can you theorize qualitativ decreases with the lower iron concentration? component of a couple of enzymes in the electron transport chain of ely why the cell yield on glucose Table P7.4 Cell Batch Growth Data for Glucose Limited/ Iron Deficient Conditions Cell Concentrations (gdw/L)Iron Concentrations (ppb) Initial Glucose (gr/L) Final 0.9 0.8 0.55 1.8 1.7 1.3 1.1 Initial 1,484 72 1.7 5,265 335 61 20 Final 1,210 23 Initial 0.009 0.0085 0.0075 0.05 0.05 0.05 0.05 2 761 47 0 0 4 ppbs parts per billion or ?g/kg

Explanation / Answer

Labile iron is an important controlling factor for the proper body functions.

1. It acts as a cofactor for many proteins like hemoglobin to carry out the oxidation and reduction reactions and hence fluctuates between its ferrous and ferric state which helps in maintaining homeostasis in the body.

2. Regulation of reactive oxygen species

These factors contribute to cellular metabolism and hence iron deficiency may lead to lesser glucose concentration in the cells as the metabolism of the cells are hampered.

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