When Sanger sequencing is used, genomic fragments must be cloned into vectors us

Question

When Sanger sequencing is used, genomic fragments must be cloned into vectors using a process known as whole-genome shotgun sequencing. Review the steps of whole-genome sequencing by completing each sentence. short During whole-genome - rto trgnerts. samples must be firstsequencing, eBook aligned Next, the fragments must be for random into that results in a sequencing cut long shotgun that The data generated result in short, nucleotide sequences called must beo to produce a DNA sequence This process of DNA sequencing isbecause of the many DNA sequencing reads that must be evaluated contigs next generation dficut RNA DNA

Explanation / Answer

During whole genome shotgun sequencing, whole genome sequencing sample must be first cut int fragments.

Next , the fragments must be cloned   into plasmid that results in a contigs for random sequencing.

The data generated results in short nucleotide sequence called libray that must be aligned to produce a continuous DNA sequence.

This process of DNA sequence is difficult because of the many discontinuous DNA sequence reads that must be evaluated.

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