TUU A. bacteria 5ml Water 8 ml Water Tube 1 Tube 2 Tube 3 1. 2 mL of A. bacteria

Question

TUU A. bacteria 5ml Water 8 ml Water Tube 1 Tube 2 Tube 3 1. 2 mL of A. bacteria is added to tube 2. What would the dilution factor of tube 2 be? Dilution foctor of tube 1-1. (2 points) After inoculating 150 ?L from Tube 2 onto a plate, 220 colonies grew. What would the original cell density (OCD) be? Consider DF of the tube 2- 0.01. (Give your answer in CFU/mL) 2. If you plated 0.02mL of a sample from Tube 3, what would the final dilution factor be? Dilution factor of tube 2 is 0.01, and 1.5 ml was transferred from tube 2 to tube 3. 3. 4. What is the standard range of colonies that is neither too few to count nor too many to count? 5. Convert 0,0065 L to ?L 6. Convert 85000000 ml to L

Explanation / Answer

Answer:

Based on the given information:

1)

2)

3)

4) Standard range of colonies that is neither too few to count nor too many to count = 30-300 colonies

5) Convert 0.0065 L to ul

6) Convert 85000000 ml to L

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