Closely related macromolecules often have many characteristics in common. For ex

Question

Closely related macromolecules often have many characteristics in common. For example, they share many of the same chemical elements and functional groups. Therefore, to separate or distinguish closely related macromolecules, we need to determine how they differ and then target or label that difference.

a) What makes RNA different from DNA?

b) If you wanted to use a radioactive or fluorescent tag to label only the RNA in a cell and not the DNA, what compound(s) could you label that is/are specific for RNA?

c) If you wanted to label only the DNA, what compound(s) could you label?

Explanation / Answer

Ans A): RNA can differ from DNA with two aspects

1) RNA has ribose as pentose sugar but DNA has Deoxyribose as pentose sugar

2) RNA has Uracil and cytosine as pyramidine bases but DNA has Thymine and Cytosine as pyramidine base

Ans B): RNA differentiating with DNA with roobose sugar and Uracil base, therefore if you want to useradioactive or fluorescent tag to label only RNA in the cell you should label either ribose sugar or Uracil pyramidine base because these are specific for RNA.

Ans C): If you want to label only the DNA, you should label the deoxyribose sugar or Tymine pyramidine base because these are specific for DNA

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