39. Protein kinase A (PKA) forms a heterotetramer that consists of two regulator

Question

39. Protein kinase A (PKA) forms a heterotetramer that consists of two regulatory (R) and two catalytic (C) subunits. Binding of CAMP activates the enzyme by releasing the catalytic subunit from the regulatory dimer. How many bands will you observe if you carry out electrophoresis of PKA at the following conditions, (A) SDS-PAGE, (B) non-denaturing gel, (C) non- denature gel in the presence of cAMP? 4x CAMP ?A) 2; (B) 1: (C) 2 CT(A) 2; (B) 2; (C) 2 e: none of the above 40. With the information provided in question 39 about PKA how does cAMP change the KM of PKA? a. increase predict b. decrease no change cannot

Explanation / Answer

A39) it should be B)2,1,2 as stated by you.

A40) I also think the Km should decrease as it is the reciprocal of affinity of binding the substrate to enzyme(cAMP). So it should decrease to increase binding with cAMP.

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