e Okazaki fragment 6. The role of DNA polymerase I in DNA replication is to a. S
ID: 253749 • Letter: E
Question
e Okazaki fragment 6. The role of DNA polymerase I in DNA replication is to a. Synthesize the leading strand b. Fill in the gaps between Okazaki fragments on the lagging strand c. Replace the RNA primers with DNA d. Seal the nicks between fragment eBand C In nature, R factors are usually transferred from one bacteria to another using 7. a. Transformation b. Conjugation c. Transduction d. Transposition e. All of the above 8. An operon that encodes the genes for the utilization of maltose as a carbon source would most likely be a. An inducible operon b. A repressible operon c. A constitutive operon d. A regulatory operon 9. Which type of mutation would be least likely to be harmful to the organism? a. Frameshift b. Nonsense c. Missense d. Silent e. Impossible to determine 10. Which of the following does not require a cell or virus to transfer DNA into a bacterial cell? a. Transduction b. Transformation c. Conjugation d. Transposition e. All of the aboveExplanation / Answer
6) DNA polymerase I has 5'->3' polymerase, 3'->5' exonuclease & 5'->3' exonuclease activity. By its 5'->3' exonuclease activity it removes primers that laid by primase & replace with DNA nucleotides in the 5'->3' direction by its 5'->3' polymerase activity.
Synthesis of leading strand is primary activity of DNA polymerase III. Okazaki fragments are joined by DNA ligase.
Thus the correct option is c.
7) R factors or Resistance factors are plasmids that confer antibiotic resistance on bacteria. They have genes that are capable of modifying antibiotic. Mainly they are conjugative plasmids,i.e., they spread through conjugation; but they can also taken up less commonly by transformation & viral transduction. But as they usually transfer through conjugation, the correct option will be b.
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