caused 6.12 Shown here are two duplex DNA molecules that are single-stranded at

Question

caused 6.12 Shown here are two duplex DNA molecules that are single-stranded at each end. (The single-stranded ends are called "overhanging ends."). Suppose we put these two DNA molecules into a test tube with all the components necessary for DNA replication and incubate the mixture. Will there be any polymeriza- tion reaction? If so, show the resulting products. If no reaction takes place, explain why not. dicated panying 5-ATGGATCCTTATAAC 3' 5- CTAGTACTGGTGC-3 3- CCTAGGAATATTGAT-5' 3'-GAAGATCATGACCA 5 indicate

Explanation / Answer

if all the components are present which are required for replication then only that DNA will amplify which has 5' overhang because DNA synthesis process is 5'-3' direction only. so DNA 1 will amplify but only 2 bases will be added and no further addition will take place. in DNA 2 we have 3' overhang and no template strand so it will not amplify.

so the product of DNA 1 will be

5' ATGGATCCTTATAACTA 3'

3' TACCTAGGAATATTGAT 5'

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