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6) Okazaki fragments a) supercoil removal b) RNA primer synthesis c) 3\' to 5\'e

ID: 254077 • Letter: 6

Question

6) Okazaki fragments a) supercoil removal b) RNA primer synthesis c) 3' to 5'exonuclease activity d) lagging strand e) single-strand breaks 7) heteroduplex DNA a) single-strand breaks b) unidirectional replication c) strand invasion d) leading strand e) phosphodiester bonds at DNA nicks 8) DNA ligase a) single-strand breaks b) unidirectional replication c) strand invasion d) leading strand e) phosphodiester bonds at DNA nicks 9) When this molecule is synthesized, both strands of a DNA molecule are used as a templat a) RNA only b) DNA only c) d) both RNA and DNA neither RNA nor DNA 10) This molecule is synthesized using triphosphate nucleotides as a substrate for a polymerase enzyme that forms phosphodiester bonds. a) RNA only b) DNA only c) both RNA and DNA d) neither RNA nor DNA 11) This molecule is synthesized using nucleotides containing the bases adenine, guanine, cytosine, and uracil. a) RNA only b) DNA only c) both RNA and DNA d) neither RNA nor DNA

Explanation / Answer

6. Option d is correct. During DNA replication, both strand works as template strand. The formation of new strand on one template strand is continous while on another template it is discontinous. The discontinous fragments are known as Okazaki fragment which rejoins later with the help of an enzyme, DNA ligase.

7. Option a is correct. Heteroduplex DNA is formed by breaking of one strand of two different DNA molecules to establish recombination leading to formation of heteroduplex DNA molecule.

8. Option e is correct. DNA ligase is an enzyme which joins two DNA strand together by the formation of phosphodiester bond.

9. Option b is correct. During replication, both parental strands work as template strand for the synthesis of new strands.

10. Option c is correct. Both RNA and DNA synthesis require triphosphate nucleotides and polymerase enzyme.

11. Option a is correct. Uracil is found only in RNA not in DNA.

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