1.) If you are given the following DNA segment, set up an appropriate PCR reacti

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1.) If you are given the following DNA segment, set up an appropriate PCR reaction: 5' ATGCGCGCGC GATGCCGCGC GCGTAAAAAA3' Circle one choice in each category (A-E) that seems most appropriate. A.) Forward primer would be: -TACGCG, ATGCGC, GATGCC, AUCGCG B.) Reverse primer would be: AATGCG, TTTTTT, TTACGC, TACGCG C.) Melting temperature would be: -5°C, 32°C, 52°C, 72°C D.) Sequencing option would be: 454 pyrophosphate, shot-gun, Sanger, restriction enzyme analysis E.) Cycles would be: -1 cycle, 2 cycles, 35 cycles, 3500 cycles

Explanation / Answer

5'- ATGCGCGCGC GATGCCGCGC GCGTAAAAAA -3''

3'- TACGCGCGCG CTACGGCGCG CGCATTTTTT -5'

A. TACGCG

B. TACGCG

C. 52°c

D. Sanger

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1.) If you are given the following DNA segment, set up an appropriate PCR reacti

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