suppose that, in an E.coli cell, DNA polymerase III makes a rare error and inser

Question

suppose that, in an E.coli cell, DNA polymerase III makes a rare error and inserts a G opposite an A residue at a position 750 bp away from the nearest GATC sequence. The mismatch is accurately repaired by the mismatch repair system.

1. How many phosphodiester and phosphoanhydride bonds are expected during this repair? _______ bonds

2. ATP molecules are consumed in this process. Which enzyme(s) use ATP?

a) exonuclease I

b) the UvrD helicase

c) exonuclease VII

d) RecJ nuclease

e) the MutL-MutS complex

Explanation / Answer

Ans 2. ATP molecules are consumed in this process. UvrD helicase ( also called helicase II) that help to unwind the DNA to allow the release of the single strand between the two cuts.

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