A biotechnology firm wants to produce a strain of giant Drosophila so that genet

Question

A biotechnology firm wants to produce a strain of giant Drosophila so that genetics students do not strain MaP d their eyes looking at tiny flies. A homone called shorty substance P (SSP) normally inhibits growth in the flies. The enzyme runtase synthesizes SSP from the compound XSP in a single-step catalytic reaction A researcher isolated and sequenced runtase cDNA, but they do not know the location of the runtase gene in the Drosophila genome. Initial attempts to delete, inactivate, or otherwise mutate this gene failed Therefore, the researcher tries gene augmentation, or adding new genes to a cell Which of the follow ing gene augmentation methods could produce giant flies? O Create transgenic flies that express high levels of SSIP O Increase runtase gene expression to increase SSP production. O Insert a gene that expresses high amounts of XSP, which would inhibit runtase Insert a gene that expresses dsRNA for SSP, which would invoke the RNAi pathway and shut down SSP expression Insert a gene that expresses dsRNA for runtase, which would invoke the RNAi pathway and shut down runtase expression

Explanation / Answer

Answer - Insert a gene that expresses dsRNA for runtase, which would invoke the RNAi pathway and shutdown runtase expression.

Runtase will catalyse the XSP to synthesis SSP. The SSP is inhibits the growth of the files. So we need to inhibit the the enzymes which responsible for this process. dsRNA which inhibit this process so we are inserting this gene.

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