Question 3 1 pts You and a classmate are both doing ChlP for the same factor. In
ID: 255038 • Letter: Q
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Question 3 1 pts You and a classmate are both doing ChlP for the same factor. In the shearing step, however, you get very different results. His shearing/size distribution centers around 1000 bp whereas yours is around 300 bp on average. How does this influence the ChIP-seq results? Your peaks will be tighter (less wide) and your signal to noise on motif finding will be better His peaks will be tighter (less wide) and his signal to noise on motif finding will be better His ChlP-seq will completely fail and he will get no reads You will get the same results, it is ChIP for the same TF D Question 4 1 pts You observe differential expression of a gene between two individuals. A close look at their genomes indicates there is a single nucleotide polymorphism (SNP) close to the 5' end of the gene and a second SNP approximately 15 kb away. Which SNP caused the differential expression? It is impossible to know from this information. Neither, the differential expression HAD to be caused by something else. The distal (15kb away) SNP The proximal (promoter) SNP.Explanation / Answer
Question 1
Answer : Your peaks will be tighter and your signal to noise on motif finding will be better
Shearing distribution centres of 300bp produce sharper and less wider peaks than that of 1000bp, therefore the shearing distribution centres of 300bp produce better peaks and results.
Question 2
Answer : " it is impossible to know from this information"
A difference in a single nucleotide at a specific position in a genome is known as Single Nucleotide Polymorphism. This is the reason for most genetic variability in a population. These snp's are responsible for point mutations.
In order to know which SNP is responsible for differential expression, one should check out for the proteins expressed by that gene. If the proteins produced perform same function even though there is a difference in the amino acid sequence due to SNP, then the SNP is known as synonymous. If the protein produced is different and performed a different function than the original, then the SNP is known as non-synonymous.
By studying the variation in the protein structure one xan identify which Snp is responsible for mutation but not by it's sheer location.
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