e he lternative that best completes the statement or answers the question. 1) Wh

Question

e he lternative that best completes the statement or answers the question. 1) Which enzyme would cut this strand of DN? GCATGGATCCCAATGC 1) A) Enzyme EcoRI Recognition GIAATTC CTTAAtG Recognition GIGATCC CCCTAGG Recognition AJAGCTT TTCGAtA Recognition ICTGCIG GtACGTC Recognition B) Enzyme BamHI C) Enzyme Hindlll D) Enzyme Pst E) Enzyme HaeIII CCGG 2) An advantage of synthetic DNA over genomic or cDNA is the ability to A) obtain genes that lack exons. B) isolate unknown genes. C) make DNA from cellular RNA and the enzyme reverse transcriptase D) obtain genes that lack introns. E) insert desired restriction sites into the DNA sequence. Table 8.3 Amino Acids Encoded by the Human p53 Gene Bladder cancer his leu thr proalualuys ser ala val thr ala leu trp ly lyalna aspglu val y ply lu jata Lung cancer A Melanoma Prostate cancer his leu thr pro gluglu ye ser ala Breast cancer his leuthr pro glu glu val cys arg tyr cys pro val ala leu trp arg ala ed on the information in Table 8.3, prostate cancer is probably the result of which kin ation? )missense

Explanation / Answer

The enzyme BamHI would cut this strand of DNA since the recognition site of BamHI ie.GGATTC is present inside the DNA it is a 6 no cutter and it also generates the sticky ends in DNA.

2)The option e is correct because in synthetic DNA the desired gene fragment can be inserted inside the genome and it can be used as a Marker to map the gene.Like antibiotics resistance gene can be inserted inside the gene and a recombinant gene can be made.

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