EXAM # 2 BIO 420 1. Mass spectrometers are used to a. transport a molecule again
ID: 255849 • Letter: E
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EXAM # 2 BIO 420 1. Mass spectrometers are used to a. transport a molecule against its concentration gradient. b. couple the energy of ATP hydrolysis to a configurational change c. transport H across a membrane against a pH gradient. d. identify proteins by mass/ratio peptide analysis as part of protcomic procedures. 2. In 2010, scientists developed a series of overlapping oligonucleotides that extended a full 1.08 million base pairs and used it to develop the first complete organism using a synthetic genome. The newly created cells had normal growth and morphology In addition to the synthetic genome, why did the investigators also included a gene that confers resistance to tetracycline? a. By including an exogenous gene, they could confirm propagation of the genome by PCR b. The tetracycline resistance gene increases the genomic stability of genomes in prokaryotes. c. The tetracycline resistance gene destabilizes the genome of the host DNA allowing the novel synthetic genome to survive. d. The tetracycline resistance gene allows the selection of only the cells that are resistant to tetracycline and thus cells that survive selection carry the novel synthetic genome. 3. DNA is a double helix with a. 3.4 base pairs per complete turn of the helix. b. 10 c. 20 d. 34 4. In Meselson and Stahl's experiment, cells were grown in media containing 1N in place of 1N, and then the cells' DNA was separated by equilibrium centrifugation. This experiment demonstrated that DNA a. is replicated by DNA polymerase. b. replication is conservative. d. forms double helices by means of hydrogen bonding between base pairs. The human genome contains approximatelybillion base pairs. 01 C. 6. A codon is a a. region of DNA coding for one protein. b. sequence of three nucleotides on a tRNA that binds to an mRNA. c. sequence of three nucleotides on an mRNA that binds to specific tRNAs. d. sequence of three nucleotides on the coding strand of DNA. 7. Because the genetic code usually has more than one codon for each amino acid, the code is said to be a. redundant. b. wobbly c. a template.Explanation / Answer
1. Identify proteins by mass/ratio peptide analysis
2. For selection, answer is d
3 . 34
4. Replication is semi conservative
5. 3 billion base pairs
6. C
7.redundant
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