ownloads/lest%2003%20 2018.pdf (c) What type of secondary structure would you ex
ID: 256021 • Letter: O
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ownloads/lest%2003%20 2018.pdf (c) What type of secondary structure would you expect residues 40 to 50 to adopt? Why? (2 points) NAME: Panther #: Question 2 The biotech company you are working for wants to develop a DNA-based detection system for a pathogenic DNA virus. As supervisor, you tasked your intern with designing a short DNA oligonucleotide that would Watson-Crick base-pair with this virus' single-stranded DNA at physiologic temperature (37°C), Your intern came up with the following three candidate sequences, all of which are fully complementary to various sections of the viral DNA target A: 5-GAGCTACTTATCT-3 B: 5'-GCTA-3 C: 5-CAGCAACGTTGCTG-3 You reject two of these sequences. Which two? Explain your reasoning in each case. (4 points)Explanation / Answer
Q.2
The primer must be 15 to 22 bp long.
The primer must not self anneal.
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As you could see in the DNA sequence of Option B,
5' - GCTA - 3'
The length of the primer is very short to be used.
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Then from the Option C, you could observe that the primer self anneal.
5' - C A G C A A C
3' - G T C G T T G
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Hence only the Primer sequence of Option A could be used for the study.
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