5. In your lab, you have several cell lines, some of which are normal, some of w

Question

5. In your lab, you have several cell lines, some of which are normal, some of which are transformed
(cancerous). While preparing several tubes of cells for sterile cell culture, the ethanol used to
disinfect the outside of the tubes also washed off the labels. The only option to identify the cell
lines was to experimentally test each of them. Please answer the following questions (4 points
total). Name 2 different assays (1 point each) that can be used to distinguish between normal and
cancer cells and briefly describe the results for each cell line if they were A cancerous or B normal
cells (0,5 points each).
A. Assay 1 _____________________________
Results:
i. Cancerous behavior – _____________________________
ii. Normal behavior – _____________________________
B. Assay 2 _____________________________
Results:
i. Cancerous behavior – _____________________________
ii. Normal behavior – _____________________________
6. An infection in the peritoneum of a lab rat elicited the response and infiltration of neutrophils. The
animal was treated as below and the recruitment of neutrophils to the peritoneum (elicited
peritoneal lymphocytes) was examined. Statistically significant p values, lower than 0.05, from the
statistical analysis of antibody-treated cells versus saline were marked with an asterisk ‘*’. Answer
the following questions that pertain to the graph below.

A. What is the effect, if any, of using an antibody against selectin to the recruitment of lymphocytes
to inflamed peritoneum? (2 points)
B. What is the effect, if any, of using purified IgG (nonspecific antibody)? Choose from: increase,
decrease, no effect) (1 point)
___________________
C. Why is purified IgG (nonspecific antibody) being used? (1 point)

Explanation / Answer

5 A. Assay 1. Contact inhibition

Cancer cell : They loose contact inhibition and pile up one over the other

Normal cell : they do not pile up. they grow in a layer

Assay 2 : Metabolic rate

Cancer cell : cancer cells utilise more nutrients and change the pH of the cell culture media rapidly

Normal cell : Normal cells have comparatively lower metabolic rate.

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