tps/lsession.masterinbiology apter 13-Mechanisms ot Transcription pter 13 Critic
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tps/lsession.masterinbiology apter 13-Mechanisms ot Transcription pter 13 Critical Thinking Question 5 View?offset-next&assignmentProblemID; 109536587 14 of 26 Part A Consider an E dol po moter that could be recognized b a ?n, ontan ng RNA polymerase holoenzyme which of te romoter elements are always present, and which can be missing or altered? How does this E. coli promoter compare to a typical eukaryotic Pol ll promoter? Essay answers are limited to about 500 words (3800 characters maximum, including spaces) 3800 Character(s) remaining Submit vide Feedback Next >Explanation / Answer
The promoter element that are present always in promoter are -35 and -10 and spacer.The -35 element centered 45 base pairs upstream of the start point transcription,this sequence has the consensus sequence TTGACA..The -10 sequence centred 10 base pairs upstrean of the start point of transcription,this sequence element has the consensus sequence TATAAT.The spacer is a distance between the above two conserved elements is also important and is conserved 17 plus minus 1 base pairs.
the elements which could be missing or alrered are GC BOX,UP ELEMENT(ACTIVATOR AND REPRESSOR),DISCRIMINATOR.
The eukaryotic promoter differs from the prokaryotic promoter in having TATA BOX,CAAT BOX
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