2-Sticky ends are the result of A) treatment of a nucleotide sequence with DNA l

Question

2-Sticky ends are the result of A) treatment of a nucleotide sequence with DNA ligase. B) exposure of eukaryotic DNA to a prokaryotic plasmicd. C) infection of a cell with a bacteriophage D) DNA breaking down in the presence of reverse transcriptase. E) cutting by restriction enzymes"off center" in a specific nucleotide sequence 3-In double-stranded DNA, which of the following base ratios always equals 1? d.G/A 4- If a circular piece of DNA has three sites for a particular restriction enzyme, into how many fragments will that restriction enzyme cut the DNA? a.2 b.4 c.3 d.5 e.The answer cannot be predicted 5-Sequences in DNA that restriction enzymes bind to and cut are mostly a.random sequences b.symmetrical about the midpoint (palindromic) ?.not symmetrical about the midpoint d.antiparallel 6- which of the following DNA strands seve as template for mRNA transcriotion A. 3' CGGCATT.5 B. 5' GCCGTAA 7-In gel electrophoresis, the marker DNA (e-g. lambda cut with Hindill) is useful: a.for showing that DNA can stain orange with ethidium bromide b.for showing the size of any DNA band that corresponds with a marker DNA band c.for telling you when to stop the electrophoresis experiment d.for allowing you to calculate the size of any DNA band in other lanes on the same gel 8-The two polynucleotide chains in a molecule of DNA are: a. parallel b.discontinuous canticomplementary d.antiparallel 9- Endonuclease hydrolyze -on the DNA stand in the , sugar-phosphate bond a. Restriction site b. Restriction site, base-base bond c. Sugar-phosphate bond, restriction site d. Sugar-base bond, restriction site 10- DNA contains all but one of the following bases except. What is the incorrect base? a.adenine b.cytosine c-guanine d.thymine e.uracil

Explanation / Answer

2. Option E is correct

Sticky ends are created by endonucleases when they cut DNA

3. Option C is correct

4. Option C is correct

5. Option b is correct

6. Option a is correct

Both strands can be used as template for RNA polymerase, but strand oriented in the 3' to 5' direction is used a a template, so that it can transcribe in the 5' to 3' direction

7. Option b is correct

8. Option d is correct

Two strands are antiparallel to each other

9. Option c is correct

Endonucleases break the phsphodiester bonds with a polypeptide chain

10. Option E is correct

Uracil is present in RNA

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