1. What size band(s) will digestion with AlwN1 show? 2. What size band(s) will d
ID: 256762 • Letter: 1
Question
1. What size band(s) will digestion with AlwN1 show?
2. What size band(s) will digestion with HindIII show?
3. What size band(s) will digestion with both HindIII and AwlN1 show?
Hint: Tn10 insert is 1400 bp and was cloned into the HindIII site
Drdl 91 BsmBI 51 BstAPI 179 BsmBI 2683 Ndel 183 Eco01091 2674 Aatll -Zral 2617 BciVI 2542 Kasl Narl Sfol 235 Bg 245 / Fspl 256 Sspl 2501 Pvull 306 Acl 2297 BceAl 387 1 or 2686 Apol- EcoRI 396 Banll Sacl Eco53KI 402 Acc651-Kpnl 408 Xmnl 2294 Bcgl 2215 MCS Aval-BsoBI-Smal 2 TspMI-Xmal 41 Bamlll 417 bal 423 ccl-Hincil -Sall 429 BspMI- BfuI 433 Sbfl 434 Pstl 435 Scal 2177 Pvul 2066 pUC19 2,686 bp BsrDI 1935 Acll 1924 1 441 Hindll1 447 Avall 1837 NmeAllI 1822 Bgl 1813 BsaXI 659 BspOl Sapl 683 Tfil 781 lll-Pcil 806 Drdl 908 BsrDI 1753 Bmrl 1744 Ahdl 1694 Bill 1015 BseYl 1110 lwNI 1217 BceAl 1292Explanation / Answer
1. One band of 2686 bp will be shown after digestion with AlwN1 since it has only one site and hence linear DNA will be obtained after a single cut.
2. One band with 2686 bp will appear after digestion with HindIII. It will also result in formation of linear DNA from the circular form.
3. A total of two bands will show after digestion with HindIII and AlwN1 together. The size of bands will be 770 bp and 1916 bp.
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