22. You have identified an enzyme from a thermophilic bacterium that lives in ho

Question

22. You have identified an enzyme from a thermophilic bacterium that lives in hot springs which converts glucose into a com pattern baldness. You will be rich! However, when you express your enzyme in effectively treats male s production of your compound, you do not recover any compound from protein-expressing bacterial culture grown in standard laboratory condition at 37 C. a) In 2-3 sentences, explain why your enzyme is not giving you your compound under these conditions. b) Hoping to create a variant with higher activity in E. coli, you perform error- prone PCR to introduce random mutations into the gene. You isolate a more active variant, and determine that it has a very similar molecular weight but a much lower extinction coefficient at 280 nm. Explain in 2-3 sentences why this variant might be more active in E. coli.

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Answer:

22)

a) In 2-3 sentences explain why your enzyme is not giving you your compound under these conditions.

b) Explain in 2-3 sentences why this variant might be more active in E.coli.

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