puon ot Gene B QUESTION9 You have created a cell deficient (knocked out) for gen
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puon ot Gene B QUESTION9 You have created a cell deficient (knocked out) for gene A. When using western blot analysis to characterize the effects of this knockout on the total protein levels and GTP-bound state of protein 8 you obain the followirng data. Which of the following might you predict to be the function for the protein encoded by gene A? WT Gene A knockout protein B GTP-bound protein protein-specific antibodies a. Gene A might function as a phosphatase for protein B Ob. Gene A might function as ginase for protein B. Gene A might function as a ubiquitin ligase for protein 8. d. Gene A might function as a GAP for protein e. Gene A might function as a GEF for protein B ?Explanation / Answer
Answer: Option D is correct.
Explanation: The below gel which shows the level of protein B using protein-specific antibodies reveals that the overall protein level is constant in the presence or absence of gene A. So, gene A is not affecting the transcription or translation of gene B.
The above gel shows that GTP bound protein-B is enriched in the absence of gene A. This shows that gene A is required for GTP hydrolysis.
GAP is required for GTP hydrolysis.
GEF is required for the exchange of GDP with GTP.
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