PROCEDURE Procedure: Performs all experiments in teams of four) The Set Up for a

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PROCEDURE Procedure: Performs all experiments in teams of four) The Set Up for all esperiments software as follows: Plug the Biology Gas Pressure Sensor into the Go-Link connector, Go to Start Prepare the computer for data collection by opening the Biology with Computers click on Programs Find Vernier Software open Logger Pro click on File "Open" open Biology with Vernier open Exp. 68 "Fermentation Pressure" Verily the vertical asis has pressure scaled from ~90 to -130kPa (KiloPascals), and the horizontal axis has time scaled from 0 to minutes 2. Connect the stopper tubing assembly to a large test tube and ensure that all connections are .figer tight" and do not leak. A pressure readng of-99 to-103kPa should appear when the sensor is open to the atmosphere (Figure A) 3. Adjusting the Valves to the Pressure Sensor: Open the valve on the rubber stopper assembly so that it is open to the atmosphere (Figure 4A). Do this by turning the handle of the valve on the rubber stopper so that it is in a vertical position. Figure 4B shows the closed horizontal position used when monitoring the O generated by the enzyme Figure 44 Valve Setting vave open to the atmosphee e 49 Valve Setting Vaive cloved to the atimonphere Part A. The Effect of Enzyme Concentration on Reaction Rate Introduction Enzyme reactions occur because of the random collisions between substrate molecules and the enzyme due to Brownian Motion. Obviously as the concentration of enzymes changes these collisions will occur with increased frequency, but issues arise when considering that with each E-S C reaction more product is produced and the products get in the way of the enzyme bumping into the substrate. The question then becomes how can one maximize the number of collisions to continually formE-SC's? 1. Set up as above and then proceed to step 2. 2. Prepare a water bath (Figure 5) at your table for the enzyme solution to ensure that the

Explanation / Answer

Hypothesis for part A would be:

Slower reaction rates are observed when lesser concentration of enzyme and the rate of reaction will be increased as the concentration of enzyme is increased.

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