7. A gene expression microarray can be used to compare the amount of gene expres
ID: 257059 • Letter: 7
Question
7. A gene expression microarray can be used to compare the amount of gene expression in different types of cells. Hybridization of the red (experimental) and green (control) cDNAs is proportional to the relative amounts of mRNA in the samples (recall the nature of cDNA). Red indicates the overexpression of a gene and green indicates the underex cells relative to the control cells, yellow indicates equal expression in experimental and control cells, and no color indicates no expression in e experimental or control cells. pression of a gene in the experimental ither R red G green Y2 yellow N no color 21 In one experiment, mRNA from malignant tumor cells is converted into cDNA and labeled with red fluorescent nucleotides; mRNA from normal cells (control) from the same individual is converted into cDNA and labeled with green fluorescent nucleotides. The cDNAs from the cancerous and noncancerous cells are mixed and hybridized to a chip containing spots of DNA from genes 1 through 25 A. What conclusions can you make about which genes might be implicated in cancer in these cells? (0.5 pt) B. How might this information be used in developing treatment for cancer? (0.5 pt)Explanation / Answer
given,
Red- overexpression
Green- underexpression
Yellow- equal expression
No color- no expression
A. To differentiate between cancer and normal cells, we have to trace red and green (overexpression and underexpression)
Overexpressed genes- 2, 24
Underexpressed genes- 4, 12, 22
Therefore, overexpression of genes 2 and 24, and underexpression of genes 4, 12 and 22 are responsible for the onset of cancer.
B. Since the overexpression of genes 2 and 24 leads to development of cancer, reducing the expression of these two genes by using some compound might lead to cancer control. Similarly, increasing the expression of genes 4, 12 and 22 might be helpful in the same way.
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