Allele specific oligonucleotides (ASO) can be used to determine the different al
ID: 257191 • Letter: A
Question
Allele specific oligonucleotides (ASO) can be used to determine the different alleles present in a DNA sample. Single sperm, which have completed meiosis, can be used for a polymerase chain reaction (PCR). The amplified DNA is then spotted to filters and hybridized with probes that recognize single nucleotide differences. Four primers (called A, B, D, and E) were used to amplify DNA samples and ASO probes were used to determine the allele present (either allele 1 or 2). "X" indicates the allele is present and "–" indicates the allele is not present.
What are genotypes of this male's parents for A, B, D, E loci?
Number of Sperm Loci and Alleles A1 A2 B1 B2 D1 D2 E1 E2 45 46 X X-X X X X-X-X 17 19Explanation / Answer
A, D, E loci are heterozygous.
A, D, E loci are linked
What genotypes are likely to have been contributed by this male's parents?
Ans: A1 B1 D1 E2 and A2 B1 D2 E1
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