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k 1. (T or P) In eukaryotic cells,transcription of ribosomal RNA is primarily th

ID: 257263 • Letter: K

Question

k 1. (T or P) In eukaryotic cells,transcription of ribosomal RNA is primarily the function of RNA polymerase II. 2. or F RNA editing is one kind of post-transcriptional modification of RNA sequences. 3. r F) AUG is the "start" codon in protein translation. r F) The number of introns that a gene can have varies greatly from one to several hundreds 5. T or F) TFLH is a general transcription factor for eukaryotic RNA polymerase II is a complex of RNA and protein that serves to remove introns from pre-mRNA. A. Apoptosome Nucleosome D. Proteosome X7. Examine the following double-stranded DNA sequence that encodes the beginning of a protein: A-T-G-T-G-G-C-C-A-C-T-A-T-A-A-T-G-A T-A-C-A-C-C-G-G-T-G-A-T-A-T-T-A-C-T Which strand is the coding strand, and where is the information for the N-terminal end of the resulting peptide located? A. B. C. D. The top strand is the coding strand, and the N-terminus is on the right. The top strand is the coding strand, and the N-terminus is on the left. The bottom strand is the coding strand, and the N-terminus is on the right The bottom strand is the coding strand, and the N-terminus is on the left. 8. Which of the following is NOT a feature of the genetic code? A. Degenerate B. Triplet C. Non-overlapping D) Each codon represents a different amino acid In order to examine mRNA traascripts isolated from cells, one needs to convert a RNA template to DNA. Doing this conversion requires which of the following enzymes? 9. Reverse Transcriptase B. DNA ligase C. DNA polymerase D. RNA polymerase 10. Which of the following is NOT associated with mRNA processing in eukaryotes? A. addition of a 5' methyl guanosine capv B. 3 polyadenylation Removal of introns v DHistone acetylation X

Explanation / Answer

Q1) In eukaryotic cells , transcription of rRNA is primarily the function of RNA poly- II.

Answer- False.

Explanation :- Transcription of rRNA is done by RNA poly-I.(but it do not transcribe 5S rRNA, it is done by RNA polymerase III).

Q2) RNA editing is one kind of post transcriptional modificationof RNA sequences.

Answer- True.

Explanation- RNA editing is any posttranscriptional modification by making changes in a transcript sequence relative to the corresponding gene, as a result there is change in the information content of the RNA,it is not done in case of splicing and terminal processing. This is found in eukaryotes .It is a rare, and common forms of RNA processing.

Q3) AUG is the start codon In protein translation.

Answer- True.

Explanation- AUG is the start codon ,  The start codon is the site at which translation into protein sequences begins, The codon AUG is called the START codon , because it the first codon in the transcribed mRNA which undergoes translation. AUG is the most common START codon, and it codes for the amino acid methionine (Met) in eukaryotes and formyl methionine (fMet) in prokaryotes. During protein synthesis, the tRNA recognizes the START codon AUG by some initiation factors and starts translation of mRNA.

Q4) The number of introns a gene can have varies from one to several hundreds-

Answer- True.

Explanation- Introns are the noncoding regions of a RNA transcript, or the DNA encoding it, they are spliced out before the RNA molecule is translated into a protein.The number of introns varies from single to several based on the complexity of the organism.

Q5) TFIIH is a general transcription factor for eukaryotic RNA polymearse-II.

Answer- True.

Explanation- TFIIH is a general transcription factor for RNA Pol II, to the promoters of genes. It plays the role of a helicase which unwinds DNA.  

Q6) A_____ is a complex of RNA and protein that serves to remove introns from pre-mRNA.

Answer- B- spliceosome.

Explanation-  The spliceosome is a complex of snRNAs and SR protein. Spliceosome removes introns from a transcribed pre-mRNA, which is a type of primary transcript. This process is generally called as splicing. Only eukaryotes have spliceosomes.

Q7) In the D.S. DNA sequence in picture that encodes begining of a protein., which strand is coding strand and where is the information for the N terminus end of the resulting peptide located ?

Answer- B - The top strand is the coidng strand and the Nterminus is in left.

Explanation - here in the picture top strand is the coding strand as it has in the begining starting codon ATG, (5?-ATG-3?) , it goes from 5' to 3' direction, so N termius is on the left of teh top strand.

Q8) Which of the following is not a feature of genetic code-

Answer- D- each codon represents a different aminoacid.

Explanation- characteristic features of genetic code are They are triplet, non overlapping, are commaless, can degenrate, some code are start codon and some are stop codon.each code is specific for a single aminoacid, it can degenerate, or redundant, because a single amino acid may be coded by more than one codon.

Q9) To examine mRNA transcripts isolated from cells, one needs to convert a RNA template to DNA , doing the conversion requires which enzyme?

Answer- A- reverse transcriptase.

Explanation-Reverse transcriptase is the enzyme used to generate complementary DNA (cDNA) from an RNA template.

Q10) Which of the following is not associated with mRNA processing?

Answer- D - Histone acetylation does not occur.

Explanation- Histone acetylation regulates the structure and function of chromatin, whereas mRNA processing is done by three processes, they are By addition of a 5' cap to the beginning of the RNA, that is addition of 5' methyl guanosine cap, addition of a poly-A tail to the end of the RNA that is 3' polyadenylation and the cutting out of introns and attaching together the , good sequences or (exons).