12. Pretend that you discovered a gene that makes students do poorly on exams. Y
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12. Pretend that you discovered a gene that makes students do poorly on exams. Your goal is to remove it from the genome. ollowing is the template DNA of the gene. Transcribe this DNA and write out the resulting RNA left to right 5' to 3 b) (4 pts) Translate the RNA sequence above using the Genetic Code on the last page. Use the single letter code in parenthesis. This sequence is in the middle of the gene, so no start codon is necessary c) (9 pts) You developed a successful gene knockout system that can remove this gene. Now you want to test whether the above protein is expressed. What technology would you used to test whether the protein is expressed? State the technology and briefly describe how the technology works.Explanation / Answer
12a).
DNA template will be read from 3’-5’. So we must rewrite the template from right to left.
DNA template----3’-TAGGTACGCTGACTTTGTCTCAGTTGGAGA-5’
mRNA-------------5’-AUCCAUGCGACUGAAACAGAGUCAACCUCU-3’
b).
mRNA-------------5’-AUC CAU GCG ACU GAA ACA GAG UCA ACC UCU-3’
Amino acids------N’- Ile His Ala Thr Glu Thr Glu Ser Thr Ser –C’
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