1) Which statement below is incorrect? A) Transformation, conjugation, transduct
ID: 257388 • Letter: 1
Question
1) Which statement below is incorrect?A) Transformation, conjugation, transduction are three processes by which exogenous genetic material may be introduced into a bacterial cell.
B) Conjugation is on the transfer of genetic material between two bacterial cells in direct contact.
C) Transduction is about the injection of foreign DNA by a bacteriophage virus into the host bacterium.
D) Transformation" is more often used to describe viral DNA transfer in bacteria and animal eukaryotic cells.
E) Bacterial DNA instead of phage DNA randomly packaged in phage particle, then transferred to recipient host, is for generalized transduction. 1) Which statement below is incorrect?
A) Transformation, conjugation, transduction are three processes by which exogenous genetic material may be introduced into a bacterial cell.
B) Conjugation is on the transfer of genetic material between two bacterial cells in direct contact.
C) Transduction is about the injection of foreign DNA by a bacteriophage virus into the host bacterium.
D) Transformation" is more often used to describe viral DNA transfer in bacteria and animal eukaryotic cells.
E) Bacterial DNA instead of phage DNA randomly packaged in phage particle, then transferred to recipient host, is for generalized transduction. 1) Which statement below is incorrect?
A) Transformation, conjugation, transduction are three processes by which exogenous genetic material may be introduced into a bacterial cell.
B) Conjugation is on the transfer of genetic material between two bacterial cells in direct contact.
C) Transduction is about the injection of foreign DNA by a bacteriophage virus into the host bacterium.
D) Transformation" is more often used to describe viral DNA transfer in bacteria and animal eukaryotic cells.
E) Bacterial DNA instead of phage DNA randomly packaged in phage particle, then transferred to recipient host, is for generalized transduction. 61) Which statement below is incorrect? A) Transformation, conjugation, transduction are three processes by whic material may be introduced into a bacterial cell. B) Conjugation is on the transfer of genetic material between two bacter C Transduction is about the injection of foreign DNA by a bacterioph bacterium. D) Transformation" is more often used to describe viral DNA transfer i eukaryotic cells. E) Bacterial DNA instead of phage DNA randomly packaged in phage to recipient host, is for generalized transduction. 10
Explanation / Answer
D) Transformation" is more often used to describe viral DNA transfer in bacteria and animal eukaryotic cells.
Transformation is one of three processes by which exogenous genetic material may be (horizontal gene transfer) introduced into a bacterial cell fragments of DNA picked up from the environment
Transformation can occur in two ways: natural transformation and artificial transformation. Natural transformation describes the uptake and incorporation of naked DNA from the cell’s natural environment. Artificial transformation encompasses a wide array of methods for inducing uptake of exogenous DNA. In cloning protocols, artificial transformation is used to introduce recombinant DNA into host bacteria (E. coli). The most common method of artificial transformation of bacteria involves use of divalent cations (e.g., calcium chloride) to increase the permeability of the bacterium’s membrane, making them chemically competent, thereby increasing the likelihood of DNA acquisition. Another artificial method of transformation is electroporation, in which cells are shocked with an electric current, to create holes in the bacterial membrane. With a newly-compromised cell membrane, the transforming DNA is free to pass into the cytosol of the bacterium.
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