Name: Sectioe: Laboratory Exercise #g (GLO Bacterial Transformation): worksheet
ID: 257806 • Letter: N
Question
Name: Sectioe: Laboratory Exercise #g (GLO Bacterial Transformation): worksheet A 1. SYE What does the abbreviation GFP stand for7 What organism is the original source of this gene? 2. What is a genetic transformation? 3. How will you know that bacterial oels have been successfully transformed with GFP and are actively expressing the GFP gene? 4. What is a plasmid? List two genes that are carried on the pGLO plasmid. 5. What is the point of inoluding ampiailin in some of the LB agar plates? What is the point of including arabinose in some of the LB agar plates? Hirt: look at the diagram e page 61) 6. Why is a bactorium a better choice for a genetic transformation than a mouse?Explanation / Answer
(1) GFP stands for green fluorescent protein
Green Fluorescent Protein (GFP) is a protein produced by a jellyfish Aequorea victoria
(2) Genetic Transformation is a process where bacterial cells take up foreign DNA and recombined with their DNA.
(3) Cells that are transformed with GFP will fluoresce under excitation source.
(4) A plasmid is an extracellular DNA (typically a small circular DNA strand in the cytoplasm of a bacterium)
pGLO plasmid contains two reported genes- the green fluorescent protein and the ampicillin resistance gene.
(5)Those cells that are transformed with pGLO plasmid can grow on this antibiotic medium, and non transformed cells won't grow in ampicillin LB medium.
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