6. Based on the western blot experiment below, which looks at specific protein e
ID: 257927 • Letter: 6
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6. Based on the western blot experiment below, which looks at specific protein expression levels, suzgest a mechanism that explains how drug X could produce these results. Acetylaed Histoe 7. rou are interested in understanding the gene regulation of biliruoin trarelocase (BIL), a protein tha iS normalty produced in liver & Kaney celsin mice. Tou tra tar tre ??? gene not expressed in heart cel. Further experiments demonstrate that there are three repons in the promoter, labeled A, B, and C in the tigure below, that contribute to this expression pattern. Assume that a single and unique transcription factor binds to each site, such that protein Xbinds site A protein Y binds site B, protein Z binds site C. You want to determine which region is responsible for the tssue-specific expression of brl, and so you create mutations in the promoter to determine the function of each of the three regions (A B, & C). In the figure below, if the site is missing, it is mutated such that it cannot bind its corresponding transcription factor. no yes yeExplanation / Answer
6. The experiment shows that increasing the concentration of drug to 6 uM completely inhibited the protein(acetylated histone). Increasing the concentration from 1 to 5 uM sequentially reduced the protein expression but did not completely inhibit the expression. Higher acetylated histone protein expression was seen when low concentration i.e., 1 uM drug was used compared to the higher concentration of 5uM drug usage. All the concentrations did not alter the control B actin protein expression
7. Presence of site C leads to no expression of the protein in the heart as can be seen from experiment 1, 2 and 5. In experiment 1 where site A, B, and C are present but the presence of C leads to no expression in the heart. Expression in the heart is seen when no site C is present as seen from the experiment 2. Supporting these results is experiment 5 where the absence of site A and B and presence of C lead to no expression in all 3
Site A is responsible for expression in the kidney as can be seen from experiment 3,6 and 7. As can be seen from experiment 3, where the presence of site A showed expression in kidney. Supporting this is experiment 6 and 7 where the presence of site A showed expression in kidney
Site B is responsible for expression in the liver as can be seen from experiment 4,6 and 8. As can be seen from experiment 4, where the presence of site B showed expression in liver. Supporting this is experiment 6 and 8 where the presence of site B showed expression in liver.
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